• <span id="u5htm"></span>

      <s id="u5htm"><samp id="u5htm"><listing id="u5htm"></listing></samp></s>

    1. <tbody id="u5htm"><p id="u5htm"></p></tbody><progress id="u5htm"></progress>
      <dd id="u5htm"><pre id="u5htm"></pre></dd>
        <th id="u5htm"></th>
          1. <s id="u5htm"></s>

            <dd id="u5htm"><pre id="u5htm"></pre></dd>

            <button id="u5htm"></button>

            1. CUSTOM MODEL GENERATION SERVICES

              Customized Mouse Models Created Using Gene Editing

              GemPharmatech constructs more than 4000 customized mouse models per year utilizing gene editing technology. This construction capacity ranks among the best in the world. Gene editing technology is the most common gene editing technology in use today and can be used to establish knockout mice (KO), conditional knockout mice (cKO), and knockin mice (KI). 

              Compared with other technologies, such as TALEN, ZFN, or ES, gene editing is simple, fast, and low cost.

               

              Advantages:

              • High quality: Create models with no random insertions and no serial recombination

              • High efficiency: Our platform results in a higher success rate and shorter delivery cycle than traditional methods

              • Multi-modification: Target multiple loci, multiple genes, and large fragments

              • Multi-background: Models can be created on a variety of genetic backgrounds, including C57BL/6J, C57BL/6N, BALB/c, FVB, NOD/LTJ, and NCG

               

              Embryonic Stem Cell Homologous Recombination Technology for Creating Customized Mouse Models

              Embryonic Stem Cell (ES) Homologous Recombination (HR) technology is a gene editing technique that integrates foreign DNA into the genome of ES cells. It is suitable for establishing mouse models with complex editing strategies or when the DNA inserts needed are larger than gene editing technology can accommodate.


              Advantages:

              • Self-deletion of resistance genes can save 3 months in model generation time 

              • BAC shooting technology can achieve knock-in segments of 100-300kb 

              • Well-established technique in use since 2005 with more than 700 models successfully completed 

              • Models can be created on a variety of genetic backgrounds, including 129S6/SvEvTac and C57BL/6N 

               

              Creating Customized Mouse Models Using Transgenic Technology

              Transgenic technology is generally used to establish customized models for overexpressed or conditionally overexpressed mice. In transgenic technology customization, DNA is randomly integrated into the mouse genome by injecting DNA directly into a fertilized mouse egg pronucleus using microinjection technology. With the increasing efficiency of knockins (KI), most models now use the H11 or Rosa26 site for KI to create overexpressed models. 


              Comparison of transgenic technology and KI at the H11 or Rosa26 site

              Transgenic

              H11/Rosa26 Knockin

              F0 generation in as little as 8 weeks

              F1 generation in as little as 16 weeks

              Low technical difficulty

              High technical difficulty

              Different expression levels between founder lines, and expression levels over time (typically expression levels decrease)

              Same expression levels in all founder lines, and constant expression levels over time

              Random insertion site and number of copies

              Accurate insertion location and gene copy number by design

              Easy to alter or destroy the expression of other endogenous genes

              Does not affect expression other endogenous genes

              Poor genetic stability

              High genetic stability

              Large-scale work in establishing a stable line

              Produces a stable line directly for analysis


              TALK WITH OUR EXPERTS

              GemPharmatech is committed to protecting and respecting your privacy, and we'll only use your personal information to administer your account and to provide the products and services you requested from us. From time to time, we would like to contact you about our products and services, as well as other content that may be of interest to you. If you consent to us contacting you for this purpose, please click below to say how you would like us to contact you:

              In order to provide you with the content requested, we need to store and process your personal data. If you consent to us storing your personal data for this purpose, please click the checkbox below.

              You can unsubscribe from these communications at any time. For more information on how to unsubscribe, our privacy practices, and how we are committed to protecting and respecting your privacy, please review our Privacy Policy.

              偷窥小说 进击的巨人小说 执掌风云小说 烟雨蒙蒙小说